Non-targeted analysis using LC-MS/MS DIA acquisition

This tutorial walks you through the workflow for analyzing non-targeted liquid chromatography high resolution mass spectrometry (LC-MS/MS) data-independent acquisition (DIA) data starting from input file generation, to processing the data in SmartPeak, to reviewing the data in SmartPeak, to reporting the results.

../../_images/MassSpecSchemas-DIA.png

Objectives

  1. Obtaining the SOP for the workflow.

  2. Choosing a data set for demonstrating the workflow.

  3. Creating an optimized SmartPeak input templates for running the workflow.

The Workflows include

  1. Defining the transition library

  2. Processing Unknowns

  3. Reviewing the results

Todo

The rest of the tutorial.

Warning

SmartPeak supports metabolomics, lipidomics, and proteomics DIA workflows. Please contact us if you are interested in including your dataset as an example.